"November Cow" Tests Positive For

BSE Using Western Blot

      

      

by Pete Hisey on 6/13/2005 for Meatingplace.com

                

Secretary of Agriculture Mike Johanns announced during a Friday night phone conference that the animal that yielded a presumptive positive for bovine spongiform encephalopathy last November using rapid testing, and then negative for the disease after immunohistochemistry (IHC) testing was performed, has since returned a positive result under the Western blot test.

Brain samples have now been sent to OIE's BSE World Reference Laboratory in Weybridge, England, for an impartial third-party review. Results are expected later this week.

After USDA's Inspector General made the recommendation that three cattle, including the animal testing positive in November, be retested for BSE using the Western blot, samples were sent to a USDA laboratory in Ames, Iowa, where the other two animals were confirmed as negative. Experts argue about which BSE tests are most reliable, but Dr. Jean-Philippe Deslys, a world-renowned expert in BSE and other prion diseases, told Meatingplace this spring that if results are in question, Western blot generally is considered conclusive.

"We…have no information that it was an imported animal," said APHIS Chief Veterinary Officer Dr. John Clifford, who described the animal as "aged."

"It was getting up there in age and was a beef breed," he said. "That's what we're willing to release at this time."

Clifford also noted that when the Western blot was used on the U.S. animal that tested positive for the disease in December 2003, only one milligram of brain material was used and it returned a "very strong positive." In this case, far more material was used, and then concentrated, to detect the disease, and as such, Clifford typified Friday's result as a "weak positive."

Clifford emphasized "we have not confirmed a case of BSE in the U.S. at this time. We're going to do further analysis and study on this."

Johanns stressed that trading partners have assumed that BSE exists in the U.S. at some level, and if the animal is confirmed to have suffered from BSE, the discovery will not affect negotiations with those partners, most notably Japan and South Korea. "The firewalls that the USDA put in place worked," Johanns said. "The animal did not enter the food or the feed chain. Therefore, there is no risk to human health."

Jim McAdams, president of the National Cattlemen's Beef Association, said in a statement that "U.S. beef consumers should know that our beef supply is safe from BSE because we prohibit from the food supply any material that could carry the BSE agent. USDA also bans from the food supply any cattle that appear to be high-risk, including this animal. This aged animal never entered the human food or animal feed supply."

"NCBA supports getting a clear and definitive answer on this sample as quickly as possible and requests USDA Secretary Johanns take whatever steps necessary to do so," McAdams continued.

James Hodges, president of the American Meat Institute Foundation, emphasized that the news "reflects USDA's commitment to a transparent, robust testing program and consumers should not be concerned about the safety of beef."

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